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09/21/2018 13:47

Brought to light – chromobodies reveal changes in endogenous protein concentration in living cells

Dr. Nadja Gugeler Pressestelle
NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen

    Scientists at the Natural and Medical Sciences Institute (NMI) in Reutlingen and the Eberhard Karls University of Tuebingen have developed new molecular probes to monitor and quantify changes in the concentration of endogenous proteins by live-cell fluorescence microscopy.

    In a study now published in Molecular & Cellular Proteomics, Keller et al. describe how fluorescently labeled intrabodies (so-called chromobodies) are stabilized in the presence of their target proteins. Based on this newly uncovered property of chromobodies, the authors present a broadly applicable strategy to optimize chromobodies in order to visualize and measure changes of endogenous target proteins within living cells.
    Currently available procedures to measure cellular protein concentration such as quantitative immunoblotting or mass spectrometry analysis are very laborious and time consuming. Moreover, these approaches require destructive sample preparation and thus only deliver results at a single point in time.
    “Our new approach using antigen-mediated chromobody stabilization (AMCBS) allows us to continuously visualize and quantify dynamic changes of specific endogenous proteins directly within living cells – something that could not be done before”, stated Bettina-Maria Keller, the study´s first author. “We expect this approach to enable unprecedented insights into the dynamic regulation of proteins, e.g. during cellular signaling, cell differentiation, or upon drug action”.
    Publication:
    Chromobodies to quantify changes of endogenous protein concentration in living cells
    Molecular & Cellular Proteomics September 18, 2018, mcp.TIR118.000914; https://doi.org/10.1074/mcp.TIR118.000914


    Contact for scientific information:

    Prof. Dr. Ulrich Rothbauer
    NMI /Universität Tübingen
    Markwiesenstrasse 55
    72770 Reutlingen
    Email: ulrich.rothbauer@nmi.de
    ulrich.rothbauer@uni-tuebingen.de
    Tel.: +49 (0)7121 30 415


    Original publication:

    Chromobodies to quantify changes of endogenous protein concentration in living cells
    Molecular & Cellular Proteomics September 18, 2018, mcp.TIR118.000914; https://doi.org/10.1074/mcp.TIR118.000914


    More information:

    http://www.nmi.de


    Images

    Antigen-Mediated-ChromoBody-Stabilization (AMCBS)
    Antigen-Mediated-ChromoBody-Stabilization (AMCBS)
    NMI
    None

    Antigen-Mediated-ChromoBody-Stabilization (AMCBS)
    Antigen-Mediated-ChromoBody-Stabilization (AMCBS)
    NMI
    None


    Criteria of this press release:
    Journalists
    Biology, Medicine, Nutrition / healthcare / nursing
    transregional, national
    Research results, Scientific Publications
    English


     

    Antigen-Mediated-ChromoBody-Stabilization (AMCBS)


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    Antigen-Mediated-ChromoBody-Stabilization (AMCBS)


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