idw – Informationsdienst Wissenschaft
Nachrichten, Termine, Experten
Nachrichten, Termine, Experten
Abstract:
In addition to the two known initiation modes of bacterial initiation, viz. the canonical 30S-binding mode and that of leaderless mRNA, we have identified a third one, the 70S-scanning initiation, the in vivo frequency of which compares with the 30S-binding mode. In the talk I will give a general introduction of translation initiation in bacteria before I go to the specific part.
The three initiation factors show a distinct importance in each of the three initiation cases: IF3 is essential for all three modes, whereas IF1 is important for the 30S-binding mode, but in particular for the 70S-scanning initiation; up to 90% of IF1 is found on 70S ribosomes and polysomes in cell lysates. Additional features of the 70S-scanning initiation are: (i) monocistronic mRNAs and downstream cistrons in polycistronic mRNAs can be initiated by this mode; (ii) after terminating the translation of a cistron, 70S ribosomes usually do not dissociate as is generally believed, but scan up- and downstream to the next Shine-Dalgarno sequence; (iii) IF1 blocks interference of ternary complexes with the scanning process; (iv) energy-rich compounds are not required for scanning, which can be triggered by naked fMet-tRNA in model reactions.
Information on participating / attending:
Einladende:
Prof. Dr. Winfried Hinrichs, winfried.hinrichs@uni-greifswald.de
PD Dr. Heike Kahlert, Vorsitzende des Ortsverbandes der GDCh, hkahlert@uni-greifswald.de
Date:
05/27/2013 16:15 - 05/27/2013 17:15
Event venue:
Institut für Biochemie, Großer Hörsaal
Felix-Hausdorff-Straße 4
17489 Greifswald
Mecklenburg-Vorpommern
Germany
Target group:
Scientists and scholars, Students
Relevance:
regional
Subject areas:
Chemistry
Types of events:
Presentation / colloquium / lecture
Entry:
05/15/2013
Sender/author:
Sabine Köditz
Department:
Presse- und Informationsstelle
Event is free:
yes
Language of the text:
German
URL of this event: http://idw-online.de/en/event43664
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