idw – Informationsdienst Wissenschaft

Nachrichten, Termine, Experten

Grafik: idw-Logo
Grafik: idw-Logo

idw - Informationsdienst
Wissenschaft

Science Video Project
idw-Abo

idw-News App:

AppStore

Google Play Store



Instanz:
Teilen: 
15.03.2011 17:00

Saarbrücker researchers show how parental genomes are reprogrammed at the start of life in mammals

Gerhild Sieber Pressestelle der Universität des Saarlandes
Universität des Saarlandes

    An international group of scientists led by the Saarland University (Germany) in collaboration with the Babraham Institute in Cambridge (UK) and the University of Osaka (Japan) has found a new epigenetic mechanism how the genomes of mammalian embryos are reprogrammed as new life begins. This breakthrough leads to insights in the first molecular step of a complex epigenetic decoding process. The research published today in “Nature Communications” shows how the egg controls the reprogramming of DNA-methylation.

    The mechanisms behind this process remained enigmatic for the last 10 years. The researchers now show that the generation of 5-hydroxymethylcytosine is a key event in DNA-methylation reprogramming.

    Beyond the great importance for the understanding of epigenetic control of development at its very beginning, the findings have also an impact on stem cell biology and epigenetic control of aging and diseases. Many adult conditions like heart disease, diabetes, obesity, cancer and autoimmune disorders are associated with altered epigenetic regulation in the course of life.

    Jörn Walter, Professor of Epigenetics at Saarland University in Germany whose team led the collaborative work said: “In humans the DNA code is faithfully transmitted from cell to cell and over generations. However, in each new generation the epigenetic code is reset. Epigenetic changes do not alter the genes but affect their interpretation in all cells of a human body. The chromosomes of both parents undergo dramatic and essential epigenetic changes at the beginning of life. Our work deciphers a new chapter in the language and grammar of epigenetic interpretation – the knowledge of this epigenetic language will have a great impact on biomedical research.”

    Professor Wolf Reik, Scientist at the Babraham Institute, Cambridge states: “This work provides exciting new insights into how epigenomes are reprogrammed in germ cells and early embryos at the beginning of life. Elucidating these mechanisms may help us to devise better strategies for making stem cell therapies a reality. Also, erasing old epigenetic marks that are inherited from parents and grandparents may be important for healthy ageing and for preventing common diseases such as diabetes or heart disease.”

    Epigenetic modification of DNA, for example by methylation of cytosines, enables genes to be switched on or off at different times and places. The reprogramming or ‘erasure’ of such epigenetic tags after fertilisation is essential if cells of a developing embryo are to have the potential to become any type of tissue – a characteristic known as totipotency.

    Shortly after fertilisation, the egg starts the unpacking and epigenetic decoding of the sperm’s chromosomes. This maternal “dominance” over the male-derived chromosomes was discovered ten years ago by the authors. A major early event governing the reprogramming is the loss of an epigenetic chemical tag called 5-methylcytosine (5mC). However, there is a striking difference between the demethylation of maternally and paternally-derived chromosomes in the fertilised eggs; the maternal genome appears to protect itself and resist demethylation.

    The team discovered that the appearance of 5-hydroxymethylcytosine (5hmC) has a critical role in the process and is associated with structural reorganisation in the nucleus after fertilisation. Within the first hours of development, a strong accumulation of the novel modification 5hmC was seen, almost exclusively on male chromosomes. The egg produces an enzyme called Tet3, which drives the conversion of 5mC into 5hmC. The authors showed that removing this enzyme in the fertilised egg using RNAi methods dramatically decreased the conversion of 5mC into 5hmC. These findings also link the loss of 5mC, first observed by the authors 10 years ago, to its conversion to 5hmC. The authors also discovered a ‘protection factor’ a protein called PGC7, which guards maternal chromosomes against the modification, while allowing “decoding” the paternal DNA-methylation tags by modification into 5hmC. Without PGC7, the methyl groups on maternal chromosomes become accessible for hydroxylation.

    While the precise biological role of the large-scale conversion of 5mC into 5hmC remains unclear, the insights are likely to have wide implications for our general understanding of epigenetic reprogramming. It is known that external factors in the environment, or for example in our diet, may have consequences later in life or on future generations. Epigenetics is now established as the ‘integrator’ between the environment and the genome so understanding how epigenomes are modified is key to understanding the mechanisms underpinning lifelong health.

    The work was supported by a grant from the Deutsche Forschungsgemeinschaft DFG, an EMBO long-term Fellowship and by the EPIGENOME Network of Excellence.

    Contact details:
    Prof. Dr. Jörn Walter
    Email: j.walter@mx.uni-saarland.de
    Laboratory of EpiGenetics
    Campus, Building A2 4
    Postbox 151150
    D-66041 Saarbrücken, Germany
    Tel.: +49 (0)681 302 4367 / 2425, Fax.: +49(0) 681 302 2703
    http://www.uni-saarland.de/fak8/genetik/

    Prof. Wolf Reik, PhD, MD
    Email: wolf.reik@bbsrc.ac.uk
    The Babraham Institute
    Babraham Research Campus
    Cambridge CB22 3AT
    United Kingdom

    Prof. Toru Nakano, MD
    Medical University Osaka, Japan
    Email: tnakamura@patho.med.osaka-u.ac.jp
    Osaka University
    Department of Pathology
    Japan

    Publication details:
    „5-hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming”
    Mark Wossidlo, Toshinobu Nakamura, Konstantin Lepikhov, C. Joana Marques, Valeri Zakhartchenko, Michele Boiani, Julia Arand, Toru Nakano, Wolf Reik and Jörn Walter.
    The DOI for this paper will be 10.1038/ncomms1240. Once the paper is published electronically, the DOI can be used to retrieve the paper by adding it to the following URL: http://dx.doi.org/

    Fotolink: http://www.uni-saarland.de/pressefotos

    Epigenetic mechanisms are at the heart of developmental biology, orchestrating the formation of many different tissues and organs from a fertilised egg. Almost all cells in an individual have exactly the same genetic material, yet behave very differently depending on which organs they comprise. It is 25 years since scientists first suspected that there might be heritable biological information separate from the DNA sequence – another inheritance ‘code’. Epigenetic regulation enables the fine tuning of our genes and their expression in different places at different times, leading to the amazing complexity we see in humans despite the relatively small number of unique genes.


    Bilder

    Female and male-derived chromosomes of a fertilized egg of a mouse. The male-derived chromosomes appear red – their genom is already reprogrammated totally. The female-derived chromosomes (blue) are protected against the decoding process. Foto: Uni – Epigenetik Walter.
    Female and male-derived chromosomes of a fertilized egg of a mouse. The male-derived chromosomes app ...

    None


    Merkmale dieser Pressemitteilung:
    Journalisten, Wissenschaftler
    Biologie, Chemie, Medizin
    überregional
    Forschungsergebnisse, Kooperationen
    Englisch


     

    Hilfe

    Die Suche / Erweiterte Suche im idw-Archiv
    Verknüpfungen

    Sie können Suchbegriffe mit und, oder und / oder nicht verknüpfen, z. B. Philo nicht logie.

    Klammern

    Verknüpfungen können Sie mit Klammern voneinander trennen, z. B. (Philo nicht logie) oder (Psycho und logie).

    Wortgruppen

    Zusammenhängende Worte werden als Wortgruppe gesucht, wenn Sie sie in Anführungsstriche setzen, z. B. „Bundesrepublik Deutschland“.

    Auswahlkriterien

    Die Erweiterte Suche können Sie auch nutzen, ohne Suchbegriffe einzugeben. Sie orientiert sich dann an den Kriterien, die Sie ausgewählt haben (z. B. nach dem Land oder dem Sachgebiet).

    Haben Sie in einer Kategorie kein Kriterium ausgewählt, wird die gesamte Kategorie durchsucht (z.B. alle Sachgebiete oder alle Länder).