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Nachrichten, Termine, Experten
Nachrichten, Termine, Experten
Abstract
O-carbamoylation has been observed in a variety of secondary metabolites, including antibiotics as diverse as cephalomycin, novomycin and nebramycin, rhizobial nodulation factors (that directs legume host specificity), and saxitoxin, responsible for paralytic shellfish poisoning. As with the aspartate and ornithine N-carbamoyltransferases (CTases) of primary metabolism, carbamoyl phosphate acts as carbamoyl donor; in contrast to the latter enzymes, however, the secondary metabolite O-CTases possess a hitherto intriguing requirement for ATP. The 6”-O-CTase TobZ, responsible for biotransformation of tobramycin to form nebramycin 5’, comprises two modular domains, each harbouring an active centre. The C-terminal YrdC-like domain contains a binding site for carbamoyl phosphate, whilst the N-terminal ASKHA domain binds nucleotides in a metal ion dependent fashion and strongly resembles the iron metallo-ATPase Kae1. Occupation of the carbamoyl phosphate site triggers binding of Mg2+-ATP, leading to carbamoyladenylate formation. We propose that the activated intermediate is channelled to the metal-site, where the carbamoyl moiety is transferred to the tobramycin acceptor hydroxyl group. Conservation of modules within the hydrogenase maturation factor HypF as well as components of the ubiquitous threonylcarbamoyladenosine (t6A) tRNA modification apparatus suggest analogous reaction paths and point to an ancient origin of these proteins.
Information on participating / attending:
Einladender: Prof. Dr. Uwe Bornscheuer, Institut für Biochemie, Universität Greifswald, Felix-Hausdorff-Straße 4, 17487 Greifswald
Telefon 03834 86-4367, uwe.bornscheuer@uni-greifswald.de
Date:
04/12/2012 17:15 - 04/12/2012
Event venue:
Großer Hörsaal
Felix-Hausdorff-Straße 4
17489 Greifswald
Mecklenburg-Vorpommern
Germany
Target group:
Scientists and scholars, Students
Email address:
Relevance:
regional
Subject areas:
Chemistry
Types of events:
Presentation / colloquium / lecture
Entry:
03/14/2012
Sender/author:
Sabine Köditz
Department:
Presse- und Informationsstelle
Event is free:
no
Language of the text:
German
URL of this event: http://idw-online.de/en/event38952
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