idw – Informationsdienst Wissenschaft
Nachrichten, Termine, Experten
Nachrichten, Termine, Experten
Abstract: Transaminases are a widespread and diverse family of pyridoxal phosphate requiring enzymes. We are using various transaminases to create chiral amines and now have over 100 different transaminases including an (R)-specific synthetic fungal transaminase. We have used transaminases and transketolase to make chiral amino diols and have constructed dual enzyme systems in vitro and in vivo. The structures of (S) and (R)-specific transaminases give some insights into the active site and specificity.
Alkaloids are a large and diverse family of nitrogen-containing compounds, many of which are used as pharmaceuticals and they represent a huge repository of functional chemical space. We are using the enzyme (S)-norcoclaurine synthase (NCS) which carries out the key first coupling step in the pathway that generates the tetrahydroisoquinolines. The enzyme catalyses a Pictet-Spengler reaction between arylethylamines and aldehydes, generating a chiral centre. Synthetic versions of the enzyme from several plant species have been designed and expressed in E. coli. The mechanism of the recombinant NCS is being elucidated and mutations of the Coptis japonica and Thalictrum flavum NCS enzymes have been used to make novel benzyl and aliphatic tetrahydroisoquinolines.
Information on participating / attending:
Einladender: Jun.-Prof. Dr. Matthias Höhne
PD Dr. Heike Kahlert, Vorsitzende des Ortsverbandes der GDCh
Date:
12/09/2013 16:15 - 12/09/2013 17:15
Event venue:
Institut für Biochemie, Großer Hörsaal
Felix-Hausdorff-Straße 4
17489 Greifswald
Mecklenburg-Vorpommern
Germany
Target group:
Scientists and scholars, Students
Relevance:
regional
Subject areas:
Chemistry
Types of events:
Presentation / colloquium / lecture
Entry:
12/04/2013
Sender/author:
Sabine Köditz
Department:
Presse- und Informationsstelle
Event is free:
no
Language of the text:
German
URL of this event: http://idw-online.de/en/event45740
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