idw – Informationsdienst Wissenschaft
Nachrichten, Termine, Experten
Nachrichten, Termine, Experten
Abstract:
The bacterial cell envelope has a complex structure and must be enlarged when the cell grows and divides. Gram-negative bacteria have in their periplasm a mainly single-layered peptidoglycan sacculus that protects the cell from lysis due to the turgor and that is required to maintain cell shape. During growth and cell division the sacculus is enlarged by the coordinated activities of peptidoglycan synthases (penicillin-binding proteins, PBPs) and hydrolases, which presumably form multi-enzyme complexes. The molecular mechanisms of peptidoglycan growth and its regulation are poorly understood.
Cytoskeletal proteins and associated cell morphogenesis proteins control peptidoglycan synthesis from inside the cell, within large cell envelope assemblies called elongasome and divisome. Recent work showed that peptidoglycan growth is also regulated from outside the sacculus in Escherichia coli and likely other Gram-negative bacteria. The outer membrane lipoproteins LpoA and LpoB are required for the functioning of the main peptidoglycan synthases, PBP1A and PBP1B, respectively. Lpo proteins interact with their cognate PBP and activate the transpeptidase function in vitro. The Lpo-interaction occurs with small, non-catalytic domains that have co-evolved with the outer-membrane activators. Presumably, Lpo proteins regulate peptidoglycan synthesis form outside the sacculus to maintain a homogenous peptidoglycan surface density and thickness, and to fine-tune peptidoglycan growth rate. The latest data on the activation of PBPs by Lpo proteins will be presented. PBP1A-LpoA are mainly active during cell elongation, and PBP1B-LpoB are members of the divisome and provide the main peptidoglycan synthesis activity during cell division.
Ort: Hörsaal Ost, Friedrich-Ludwig Jahn-Straße 15 A, 17489 Greifswald
Zeit: Donnerstag, 12.06.2014, 17.15 Uhr
Information on participating / attending:
Kontakt: Prof. Dr. Sven Hammerschmidt
Interfakultäres Institut für Genetik und Funktionelle Genomforschung
Abteilung Genetik der Mikroorganismen, Greifswald
Friedrich-Ludwig-Jahn-Straße 15 A, 17489 Greifswald, Tel. 03834 86-4161
sven.hammerschmidt@uni-greifswald.de
Date:
06/12/2014 17:15 - 06/12/2014 18:15
Event venue:
Hörsaal Ost
Friedrich-Ludwig-Jahn-Straße 15 A
17489 Greifswald
Mecklenburg-Vorpommern
Germany
Target group:
Scientists and scholars, Students
Relevance:
regional
Subject areas:
Biology
Types of events:
Presentation / colloquium / lecture
Entry:
06/05/2014
Sender/author:
Sabine Köditz
Department:
Presse- und Informationsstelle
Event is free:
no
Language of the text:
German
URL of this event: http://idw-online.de/en/event47521
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