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Image for: Shining a Spotlight on Polyploid Cells – a tool to uncover spatial patterns of DNA content across tissues


The iSPy pipeline at a glance to quantify nuclear ploidy. Confocal microscopy is performed with nuclear reporters and stains, nuclear segmentation with ilastik is conducted, and a machine learning method is used to classify the ploidy of each nucleus.
The iSPy pipeline at a glance to quantify nuclear ploidy. Confocal microscopy is performed with nuclear reporters and stains, nuclear segmentation with ilastik is conducted, and a machine learning method is used to classify the ploidy of each nucleus.

Source: Nicholas J. Russell

Copyright: Nicholas J. Russell

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