Lichtabhängige Wasserstoffbildung in einer verschlossenen Grünalgenkultur. Links: Wasserstoffbildung in einer verschlossenen, unter Schwefel-Mangel kultivierten C. reinhardtii-Kultur. Rechts: Unter Nähstoffmangel fließen bei Grünalgen die in der Photosynthese durch Lichtaufnahme angeregten Elektronen aus der Wasserspaltung in die H2-Bildung. Der entscheidende Schritt ist die Wechselwirkung zwischen dem aus der Photosynthesekette stammenden Ferredoxin PetF und der [FeFe]-Hydrogenase HydA1. Die Hydrogenase setzt zwei nacheinander aufgenommene Elektronen mit H+-Ionen zu Wasserstoff um, der schließlich aus der Zelle entweicht.
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