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Few animals can regenerate their spinal cord after an injury. The axolotl, a salamander endemic to Mexico, can mobilise stem cells in its spinal cord to regrow the lost tissue. An international team of scientists investigated the early stages of this process and found that stem cells synchronise over surprisingly long distances to orchestrate regeneration. Their findings are now published on the online platform eLife.
The spinal cord is an important component of our central nervous system: it connects the brain with the rest of the body and plays a crucial part in coordinating our sensations with our actions. Falls, violence, disease – various forms of trauma can cause irreversible damage to the spinal cord, leading to paralysis, sometimes even death.
Although many vertebrates, including humans, are unable to recover from a spinal cord injury, some animals stand out. For instance, the axolotl (Ambystoma mexicanum), a salamander from Mexico, has the remarkable ability to regenerate its spinal cord after an injury. When an axolotl’s tail is amputated, neural stem cells residing in the spinal cord are recruited to the injury to rebuild the tail. So far, scientists could only detect this activity a few days after the process had started.
“Four days after amputation, stem cells within about one millimetre of the injury divide three times as fast as the normal rate to regenerate the spinal cord and replace lost neurons,” explains Emanuel Cura Costa, co-first author of the study. “What the stem cells are doing in the first four days after injury was the real mystery.”
To understand what happens in the first moments of spinal cord regeneration, researchers at Argentina’s National Scientific and Technical Research Council (CONICET) and at the Research Institute of Molecular Pathology (IMP), Austria, teamed up to recreate the process in a mathematical model and test its predictions in axolotl tissue with the latest imaging technologies. Their findings, published in eLife, show that neural stem cells accelerate their cell cycles in a highly synchronised manner, with the activation spreading along the spinal cord.
Regenerating in sync: cells follow the tempo
In the uninjured spinal cord, cells multiply asynchronously: some are actively replicating their DNA before splitting into two cells to sustain growth, while some are simply resting.
The scientists’ model predicted that this could change dramatically upon injury: most cells in the vicinity of the injury would jump to a specific stage of the cell cycle to synchronise and proliferate in unison.
“We developed a tool to track individual cells in the growing spinal cord of axolotls. Different colours label resting and active cells, which allow us to see how far and how fast cell proliferation happens with a microscope,” says Leo Otsuki, postdoc in the lab of Elly Tanaka at the IMP and co-first author of this study. “We were very excited to see the match between the theoretical predictions and the experimental results.”
The way cells multiply in chorus in the regenerating spinal cord is exceptional in animals. How can cells coordinate their efforts over almost one millimetre - 50 times the size of a single cell?
A mystery signal orchestrating regeneration
“Our model made us realise there had to be one or more signals that spread through the tissue from the injury, like a wave, for the area of proliferating cells to expand,” explains Osvaldo Chara, career researcher at CONICET and group leader of SysBio at the Institute of Physics of Liquids and Biological Systems (IFLySIB). “This signal might act like a messenger and instruct stem cells to proliferate.”
The researchers suspect that this mystery messenger helps reprogram stem cells to divide rapidly and regrow amputated tissue. Their work pinpoints this signal in space and time, and paves the way to characterise it further.
“Combining mathematical models with our expertise in tissue imaging was key to understanding how the spinal cord starts regenerating,” says Elly Tanaka, senior scientist at the IMP. “The next step is to identify the molecules that promote regeneration of the spinal cord - that could have tremendous therapeutic potential for patients with spinal injuries.”
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About the IMP
The Research Institute of Molecular Pathology (IMP) in Vienna is a basic life science research institute largely sponsored by Boehringer Ingelheim. With over 200 scientists from 40 countries, the IMP is committed to scientific discovery of fundamental molecular and cellular mechanisms underlying complex biological phenomena. The IMP is part of the Vienna BioCenter, one of Europe’s most dynamic life science hubs with 2,000 employees from 70 countries in four research institutes, three universities and almost 40 biotech companies.
www.imp.ac.at, www.viennabiocenter.org
About CONICET
CONICET is the main agency that fosters science and technology in Argentina. Currently, the Council has more than 10,000 researchers, 11,000 doctoral and postdoctoral fellows, 2,600 technicians and professional support staff, and 1,500 administrative employees. They all operate across the whole country in 15 Scientific and Technological Centers (CCT), 11 research and transference centres (CIT), a Multidisciplinary Research Center and more than 280 Institutes and exclusive CONICET Centers under the scope of national universities and other institutions.
https://www.conicet.gov.ar/
elly.tanaka@imp.ac.at
Cura Costa, E., Otsuki, L., Rodrigo Albors, A., Tanaka, E. M., Chara, O.: “Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration”. eLife, 8 June 2021. DOI: : https://doi.org/10.7554/eLife.55665.
Cross-section of axolotl spinal cord. Neural stem cells are red and green in the middle, lining the ...
Leo Otsuki
IMP
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