idw – Informationsdienst Wissenschaft

Nachrichten, Termine, Experten

Grafik: idw-Logo
idw-Abo
Medienpartner:
Wissenschaftsjahr


Teilen: 
24.07.2018 12:28

Protein complex for hepatitis C-virusentry in liver cells is characterised

Dr. Jo Schilling Presse- und Öffentlichkeitsarbeit
TWINCORE - Zentrum für Experimentelle und Klinische Infektionsforschung

    Hepatitis C virus chronically infects 70 million individuals worldwide. Although the infection can be cured with antiviral drugs, this therapy does not protect from a second infection with the same virus. Moreover, the liver damage cannot always be reversed by treatment of hepatitis C. The team “Viral Interaction Proteomics” led by Gisa Gerold at the Institute for Experimental Virology at TWINCORE used a new sensitive analytical method to identify proteins in the membrane of liver cells, which mediate entry of hepatitis C virus. These results were recently published in PLOS Pathogens.

    The receptors, which viruses –and other extracellular moieties- use to enter cells, are often part of large complexes composed of many proteins. In the case of hepatitis C virus a critical receptor is CD81. It is part of a complex -the tetraspanin web-, which is embedded in the liver cell membrane. The complex binds the virus on the surface of liver cells. Its composition was now elucidated by the team of Gisa Gerold. Moreover, she and her co-workers could show how the virus is high jacking the complex to gain access into liver cells. “The protein complex deserves the name ‘complex’ as it comprises a fairly large number of proteins. It furthermore engages additional cytosolic proteins.”, says Gisa Gerold. “To understand, how virus entry works, it is not enough to look at CD81 in an isolated manner. We need to take into account, which other molecules are engaged by CD81.” And exactly that is what the method of the biochemist can do.The work revealed that the CD81 complex in liver cells consists of more than 33 proteins. Using quantitative mass spectrometry – a method, which elucidates the nature of proteins in the complex and their relative abundance – she analyzed the molecular environment of the hepatitis C virus entry receptor CD81. For 30 of the 33 interacting proteins it was unclear, whether they played a role in hepatitis C virus infection. By testing every single one of the 33 proteins for their role in infection, the researchers found two novel proteins, which are important for virus entry: calpain-5 (CAPN5) and Casitas B-lineage lymphoma proto-oncogene B (CBLB). „The functional role of CAPN5 and CBLB in hepatitis C virus entry into liver cells was previously unknown. We could thus not only map proteins within the CD81 complex, but also elucidate two novel key components of the complex that contribute to virus infection”, concludes Gisa Gerold.


    Wissenschaftliche Ansprechpartner:

    Dr. Gisa Gerold, gisa.gerold(at)twincore.de
    Tel: +49 (0)511 220027-134


    Originalpublikation:

    Bruening J, Banse P, Kahl S, Vondran FW, Kaderali L, Marinach C, Silvie O, Pietschmann T, Meissner F*, Gerold G*. (2018) Hepatitis C virus enters liver cells using the CD81 receptor complex proteins calpain-5 and CBLB. PLOS Pathogens. * these authors contributed equally


    Weitere Informationen:

    https://www.twincore.de/en/institutes/experimental-virology/rg-virus-interaction... More about Gisa Gerold's research.


    Merkmale dieser Pressemitteilung:
    Journalisten, Studierende, Wissenschaftler
    Biologie, Medizin
    überregional
    Forschungsergebnisse, Wissenschaftliche Publikationen
    Englisch


    Dr. Gisa Gerold


    Zum Download

    x

    Hilfe

    Die Suche / Erweiterte Suche im idw-Archiv
    Verknüpfungen

    Sie können Suchbegriffe mit und, oder und / oder nicht verknüpfen, z. B. Philo nicht logie.

    Klammern

    Verknüpfungen können Sie mit Klammern voneinander trennen, z. B. (Philo nicht logie) oder (Psycho und logie).

    Wortgruppen

    Zusammenhängende Worte werden als Wortgruppe gesucht, wenn Sie sie in Anführungsstriche setzen, z. B. „Bundesrepublik Deutschland“.

    Auswahlkriterien

    Die Erweiterte Suche können Sie auch nutzen, ohne Suchbegriffe einzugeben. Sie orientiert sich dann an den Kriterien, die Sie ausgewählt haben (z. B. nach dem Land oder dem Sachgebiet).

    Haben Sie in einer Kategorie kein Kriterium ausgewählt, wird die gesamte Kategorie durchsucht (z.B. alle Sachgebiete oder alle Länder).

    Cookies optimieren die Bereitstellung unserer Dienste. Durch das Weitersurfen auf idw-online.de erklären Sie sich mit der Verwendung von Cookies einverstanden. Datenschutzerklärung
    Okay