idw – Informationsdienst Wissenschaft

Nachrichten, Termine, Experten

Grafik: idw-Logo
Science Video Project
idw-Abo

idw-News App:

AppStore

Google Play Store



Instanz:
Teilen: 
15.01.2019 15:50

Velcro for human cells

Rudolf-Werner Dreier Presse- und Öffentlichkeitsarbeit
Albert-Ludwigs-Universität Freiburg im Breisgau

    Freiburg researchers engineer cellular adhesion receptors that can be controlled with light

    The ability of cells to adhere to each other and to their environment is the basis for multicellular life. Adhesion occurs via diverse receptors at the surface of cells that bind to specific ligands in their surroundings. Despite the importance of these adhesion receptors, there is a paucity of tools available for precisely controlling their interactions with the environment. To address this limitation, an interdisciplinary team of scientists from the Freiburg Signalling Research Excellence Clusters BIOSS and CIBSS have engineered an adhesion receptor and a complementary synthetic extracellular environment that can be activated by light. This system can be adapted to render other receptor–ligand interactions amenable to precise manipulation with light. The scientists have published their new optogenetic system in Communications Biology.

    Optogenetics uses light to control proteins and the cellular processes in which they are involved. “This technique has revolutionized the analysis of cellular signalling because it is non-invasive and because it allows precise spatiotemporal control of signalling processes”, says lead author of the study, Prof. Dr. Wilfried Weber. Optogenetics has been widely used to control processes inside the cells. The authors decided to bring optogenetics into the extracellular realm, specifically to test whether they could control receptor–matrix interactions using light.

    The team of researchers, which also included the groups of Prof. Dr. Gerald Radziwill and Prof. Dr. Wolfgang Schamel, focussed their attention on an important class of receptors – called integrins – that facilitate adhesion to extracellular matrix. “Although integrins have central roles in many normal biological processes, they can also promote growth and spread of cancer and have thus been explored as targets for anti-cancer therapies.” explains first author Julia Baaske. To control integrin-mediated adhesion using light, the scientists first developed an OptoMatrix coated with a light-sensitive plant protein called phytochrome B. Then they engineered an OptoIntegrin equipped with a phytochrome-interacting factor (PIF6) and expressed this receptor in cancer cells. Phytochrome B is usually found in its inactive form, but when exposed to a specific wavelength of red light it is activated and can be bound by PIF6; when exposed to infrared light it switches back to the inactive form. “The most exciting experiment was when we first shone red light on the OptoMatrix: the cells expressing OptoIntegrin immediately adhered to the matrix and activated intracellular signalling processes. Then when we used infrared light, they almost completely detached.” explains Baaske. “Essentially, we have developed light-controlled velcro for human cells”.

    The system not only allows integrin–matrix interactions to be turned on and off with high temporal precision; it also allows spatial control. Strong adherence of cells expressing OptoIntegrins only occurs at parts of the Optomatrix that have been activated with light. “This system serves as a blueprint for precise spatiotemporal control of other receptor–ligand interactions using light.” says Weber, who is also a member of the Speaker Team of the recently launched CIBSS Excellence Cluster. “This and other optogenetic and chemical control-of-function technologies that will be developed in CIBSS will give us unprecedented control of and insight into the spatiotemporal dynamics of biological signalling processes.”


    Wissenschaftliche Ansprechpartner:

    Prof. Dr. Wilfried Weber
    CIBSS – Centre for Integrative Biological Signalling Studies
    Phone: +49 (0)761 / 203 - 97654
    E-Mail: wilfried.weber@biologie.uni-freiburg.de


    Originalpublikation:

    Baaske J., Mühlhäuser W.W.D., Yousefi O.S., Zanner S., Radziwill G., Hörner M., Schamel W.W.A., Weber W. (2019): Optogenetic control of integrin–matrix interaction. In: Communications Biology. DOI: 10.1038/s42003-018-0264-7


    Weitere Informationen:

    https://www.pr.uni-freiburg.de/pm-en/press-releases-2018/velcro-for-human-cells


    Bilder

    The OptoMatrix-OptoIntegrin system: OptoIntegrin-expressing cancer cells are seen adhering to the OptoMatrix, but only to the part illuminated with red (660nm) light. Photo: J. Baaske
    The OptoMatrix-OptoIntegrin system: OptoIntegrin-expressing cancer cells are seen adhering to the Op ...

    None


    Merkmale dieser Pressemitteilung:
    Journalisten, Wissenschaftler
    Biologie, Medizin
    überregional
    Forschungsergebnisse, Wissenschaftliche Publikationen
    Englisch


     

    The OptoMatrix-OptoIntegrin system: OptoIntegrin-expressing cancer cells are seen adhering to the OptoMatrix, but only to the part illuminated with red (660nm) light. Photo: J. Baaske


    Zum Download

    x

    Hilfe

    Die Suche / Erweiterte Suche im idw-Archiv
    Verknüpfungen

    Sie können Suchbegriffe mit und, oder und / oder nicht verknüpfen, z. B. Philo nicht logie.

    Klammern

    Verknüpfungen können Sie mit Klammern voneinander trennen, z. B. (Philo nicht logie) oder (Psycho und logie).

    Wortgruppen

    Zusammenhängende Worte werden als Wortgruppe gesucht, wenn Sie sie in Anführungsstriche setzen, z. B. „Bundesrepublik Deutschland“.

    Auswahlkriterien

    Die Erweiterte Suche können Sie auch nutzen, ohne Suchbegriffe einzugeben. Sie orientiert sich dann an den Kriterien, die Sie ausgewählt haben (z. B. nach dem Land oder dem Sachgebiet).

    Haben Sie in einer Kategorie kein Kriterium ausgewählt, wird die gesamte Kategorie durchsucht (z.B. alle Sachgebiete oder alle Länder).