idw – Informationsdienst Wissenschaft

Nachrichten, Termine, Experten

Grafik: idw-Logo
Thema Corona

Imagefilm
Science Video Project
idw-News App:

AppStore



Teilen: 
16.08.2021 14:42

Bacterial toxin blinds algae

Dr. Ute Schönfelder Abteilung Hochschulkommunikation/Bereich Presse und Information
Friedrich-Schiller-Universität Jena

    University of Jena researchers have discovered a bacterial toxin that destroys the colour pigments in the eyespot of the single-cell green alga Chlamydomonas reinhardtii. Together with another toxic substance, the bacteria of the species Pseudomonas protegens not only disorientate and immobilise the green algae, but condemn them to a certain death. The researchers detected the toxin, called “protegencin”, with the help of Raman spectroscopy and natural product research. They have published the results of their study in the current issue of the journal “PNAS” (DOI: https://doi.org/10.1073/pnas.2107695118).

    The researchers led by the professor for General Botany of the University of Jena, Maria Mittag, already knew from earlier studies that an encounter with Pseudomonas protegens bacteria did not do the green algae Chlamydomonas reinhardtii any good at all. Now the deadly toxin has been identified by Prof. Mittag and doctoral candidate Vivien Hotter, together with the teams led by Prof. Jürgen Popp (Institute for Physical Chemistry of the University of Jena and Leibniz Institute of Photonic Technology) and Prof. Christian Hertweck (Institute of Microbiology of the University of Jena and Leibniz Institute for Natural Product Research and Infection Biology – Hans Knöll Institute), as well as Prof. Severin Sasso (Leipzig University).

    “The green algae have a primitive eye, called the eyespot, with which they perceive light and can orientate themselves,” explains Maria Mittag. This enables the single-cell organisms, measuring around 10 micrometres, to move towards the light, in order to have optimum conditions for photosynthesis. The eyespot contains two layers of yellowish carotenoid-rich fat droplets.
    “The eyespot carotenoids can be studied by using Raman spectroscopy,” adds Prof. Mittag. This method analyses the scattering of laser light on molecules and provides a characteristic spectrum, comparable to a spectroscopic “fingerprint”.

    Chemical “fingerprints” reveal the “murder weapon”

    With this instrument, the researchers, together with Prof. Popp’s team, set about their detective work and analysed the “Raman fingerprints” from algal cultures to which Pseudomonas bacteria had been added.

    “You can see the effect just with the naked eye,” reports Vivien Hotter. The green algal culture loses its colour almost completely overnight. “In parallel, the Raman signal for the eyespot carotenoids decreases.” This suggests that the algae lose their ability to orientate themselves in light, says Vivien Hotter. But that is not all: “We were also able to show that the toxin dissolves their cell membrane. The algae don’t survive that.”

    The researchers, in cooperation with their colleagues in the “ChemBioSys” Collaborative Research Centre, tracked down the “murder weapon” that enables the bacteria to kill the algae. The analysis of the Raman spectra showed that not only does the signal for the eyespot pigments decrease in the course of the bacterial attack, but that a new signal not previously present also appears in the spectrum. Prof. Hertweck’s team of natural product researchers used this signal to decipher the basic chemical structure of the bacterial toxin. Together with the other researchers, they ultimately succeeded in identifying the substance “protegencin” as the cause of death.

    With their basic research, the scientists in “ChemBioSys” are looking for natural substances that influence the interaction between biosystems such as microalgae and bacteria, with the aim of clarifying the underlying control mechanisms. Although microalgae contribute significantly to global oxygen production and, together with cyanobacteria, fix around 50 percent of the greenhouse gas carbon dioxide, the fundamental control mechanisms in the complex biosystems of algae and bacteria are not yet sufficiently well understood.


    Wissenschaftliche Ansprechpartner:

    Prof. Maria Mittag , Vivien Hotter
    Matthias Schleiden Institute, Area of General Botany Friedrich Schiller University Jena
    Am Planetarium 1, 07743 Jena, Germany
    Tel.: +49 (0)3641 9-49201, 9-49219
    E-mail: m.mittag[at]uni-jena.de, vivien.hotter[at]uni-jena.de


    Originalpublikation:

    Original Publication:
    Hotter V. et al. A polyyne toxin produced by an antagonistic bacterium blinds and lyses a Chlamydomonad alga. Proceedings of the National Academy of Sciences (PNAS) 2021, DOI: https://doi.org/10.1073/pnas.2107695118.


    Merkmale dieser Pressemitteilung:
    Journalisten, Wissenschaftler
    Biologie
    überregional
    Forschungsergebnisse
    Englisch


    Green algae almost completely lose their colour when the deadly bacteria have been added to them.


    Zum Download

    x

    Hilfe

    Die Suche / Erweiterte Suche im idw-Archiv
    Verknüpfungen

    Sie können Suchbegriffe mit und, oder und / oder nicht verknüpfen, z. B. Philo nicht logie.

    Klammern

    Verknüpfungen können Sie mit Klammern voneinander trennen, z. B. (Philo nicht logie) oder (Psycho und logie).

    Wortgruppen

    Zusammenhängende Worte werden als Wortgruppe gesucht, wenn Sie sie in Anführungsstriche setzen, z. B. „Bundesrepublik Deutschland“.

    Auswahlkriterien

    Die Erweiterte Suche können Sie auch nutzen, ohne Suchbegriffe einzugeben. Sie orientiert sich dann an den Kriterien, die Sie ausgewählt haben (z. B. nach dem Land oder dem Sachgebiet).

    Haben Sie in einer Kategorie kein Kriterium ausgewählt, wird die gesamte Kategorie durchsucht (z.B. alle Sachgebiete oder alle Länder).