idw – Informationsdienst Wissenschaft

Nachrichten, Termine, Experten

Grafik: idw-Logo
Science Video Project

idw-News App:


Google Play Store

30.05.2023 17:27

A rapid diagnostic test to detect multiple resistance determinants against the important carbapenem antibiotics

Dr. Nicola Wittekindt Presse- und Öffentlichkeitsarbeit
Deutsches Zentrum für Infektionsforschung

    In 2050, the number of people dying as a result of infection with antibiotic-resistant bacteria could rise to ten million, according to WHO forecasts. For targeted therapies and to prevent the spread of antibiotic resistance, rapid and accurate diagnosis of resistance is essential. A new rapid test developed by DZIF researchers in cooperation with the company Coris BioConcept now makes it possible to detect over 95 percent of carbapenem resistance in patients infected with the widespread pathogenic bacterium Acinetobacter baumannii.

    Every year at least 700,000 people die as a result of infection with antibiotic-resistant bacteria—a figure which according to WHO forecasts could rise to ten million people by 2050 without new measures to combat the development and spread of resistance. In this context, rapid and targeted diagnosis of resistance is essential to determine appropriate antibiotic therapies and limit the spread of antibiotic resistance. In 2018, DZIF scientists from the University of Cologne in cooperation with the Belgian company Coris BioConcept had developed a rapid diagnostic test (OXA-23 K-SeT®) that uses OXA-23-specific antibodies to detect in Acinetobacter baumannii the widespread OXA-23 carbapenem resistance determinant within minutes. In a recent publication, the scientists now describe the further development of the rapid test into a multi-carbapenemase assay capable of detecting over 95 per cent of carbapenem resistance in A. baumannii-infected patients.

    In addition to OXA-23, the second-generation rapid test, called RESIST ACINETO, also detects the carbapenemases OXA-40 and OXA-58, as well as the metallo-beta-lactamase NDM, another enzyme that can cleave antibiotics from the carbapenem class. "The RESIST ACINETO rapid test we co-developed screens samples for four resistance determinants simultaneously, detecting more than 95 per cent of carbapenem-resistant A. baumannii strains," says Dr Alexander Klimka, research group leader at the University of Cologne and lead author of the study. "In a patient with an A. baumannii infection, the treating physician can use a RESIST ACINETO rapid test to determine within 15 minutes whether therapy with a carbapenem would be effective or not," says Dr Paul Higgins, DZIF-scientist at the University Hospital Cologne.

    The test, which has been marketed since May 2022, is based on the detection of carbapenemase enzymes in clinical isolates by binding highly specific antibody pairs. In a similar manner to the familiar COVID rapid tests, simultaneous binding of both antibodies to the respective enzyme results in a distinct colour response. The assay is not only rapid, but also comparatively inexpensive, easy to use, and does not require a specific reader, as is often necessary with other diagnostic methods. This makes the test particularly attractive for countries and regions that do not have an advanced medical infrastructure.

    In further collaborative projects, the team now plans to develop rapid tests for the detection of other antibiotic-resistance determinants and bacterial species, as well as human pathogenic fungi and parasites. In addition, the scientists are working on modifying the sensitivity and sample preparation so that they can also be used as point-of-care tests, i.e., without prior cultivation of the patient sample. This would not only improve the individual therapy, but also enable the early isolation of colonised or infected patients in order to prevent the spread of the respective pathogen.

    Wissenschaftliche Ansprechpartner:

    Dr. Alexander Klimka
    University of Cologne

    Dr. Paul Higgins
    University Hospital Cologne


    Mertins S. et al.: Development of an immunochromatographic lateral flow assay to rapidly detect OXA-23-, OXA-40-, OXA-58- and NDM-mediated carbapenem resistance determinants in Acinetobacter baumannii, J Med Microbiol, 2023,

    Weitere Informationen: More information on the RESIST ACINETO rapid test


    RESIST ACINETO, a multi-carbapenemase rapid test that allows to simultaneously detect four bacterial resistance factors to the clinically important antibiotic carbapenem.
    RESIST ACINETO, a multi-carbapenemase rapid test that allows to simultaneously detect four bacterial ...

    Coris BioConcept

    Merkmale dieser Pressemitteilung:
    Journalisten, Wirtschaftsvertreter, Wissenschaftler, jedermann
    Biologie, Medizin
    Forschungs- / Wissenstransfer, Wissenschaftliche Publikationen


    RESIST ACINETO, a multi-carbapenemase rapid test that allows to simultaneously detect four bacterial resistance factors to the clinically important antibiotic carbapenem.

    Zum Download



    Die Suche / Erweiterte Suche im idw-Archiv

    Sie können Suchbegriffe mit und, oder und / oder nicht verknüpfen, z. B. Philo nicht logie.


    Verknüpfungen können Sie mit Klammern voneinander trennen, z. B. (Philo nicht logie) oder (Psycho und logie).


    Zusammenhängende Worte werden als Wortgruppe gesucht, wenn Sie sie in Anführungsstriche setzen, z. B. „Bundesrepublik Deutschland“.


    Die Erweiterte Suche können Sie auch nutzen, ohne Suchbegriffe einzugeben. Sie orientiert sich dann an den Kriterien, die Sie ausgewählt haben (z. B. nach dem Land oder dem Sachgebiet).

    Haben Sie in einer Kategorie kein Kriterium ausgewählt, wird die gesamte Kategorie durchsucht (z.B. alle Sachgebiete oder alle Länder).