idw – Informationsdienst Wissenschaft

Nachrichten, Termine, Experten

Grafik: idw-Logo
Grafik: idw-Logo

idw - Informationsdienst
Wissenschaft

Science Video Project
idw-Abo

idw-News App:

AppStore

Google Play Store



Instanz:
Teilen: 
19.08.2021 12:30

Flawed quality control in the brain

Dr. Stefanie Merker Presse und Öffentlichkeitsarbeit
Max-Planck-Institut für Neurobiologie

    Proteins are only able to perform their essential tasks in the body if they fold correctly into their respective, very specific 3D structure. To ensure that nothing goes wrong with the folding process, it is strictly monitored in the cell. A flawed quality control can lead, for example, to the deposition of misfolded proteins in neurodegenerative diseases such as Alzheimer's. Researchers at the Max Planck Institutes of Neurobiology and of Biochemistry have now developed a mouse line that makes the state of protein balance visible in the mammalian brain for the first time. In this way, the processes of protein quality control can now be studied in healthy and diseased neurons in more detail.

    Proteins fulfill all important tasks in our body: They transport substances, protect against diseases, support the cell and catalyze chemical reactions – to name just a few. With the building instructions in our genetic code, every protein can be produced as a long chain of amino acids. However, that's not the end of the story: in order to perform their vital functions, proteins have to fold into complex 3D structures.

    Each cell contains a whole machinery that helps proteins to fold, corrects folding errors and discards misfolded proteins. As a kind of quality control, the system thus contributes to proteostasis – the controlled function of all proteins.

    In healthy cells, this quality control works very well. With age, however, it gradually deteriorates. This can become a problem, especially for nerve cells. These cells do not renew themselves and are therefore dependent on stable protein function throughout their lives. In fact, neurodegenerative diseases such as Alzheimer's, Parkinson's or Huntington's disease have in common that certain misfolded proteins overload the quality control system and are not disposed of. These proteins accumulate, clump together and eventually form deposits in the brain tissue. Depending on the disease, this can lead to impaired memory or muscle control – with no chance of a cure so far. The ability to enhance the neurons’ quality control could thus present a promising therapeutic option.

    In order to study the quality control defects in the individual diseases in more detail, scientists led by Irina Dudanova developed a new mouse line. With these animals, the state of proteostasis can be visualized in the mammalian brain for the first time.

    The researchers introduced the protein that normally makes fireflies glow into the neurons of the mouse. Optimized to the body temperature of the beetle, the protein needs constant help to fold in "warmer" mammals. Only then can it adopt its correct structure and produce light. In order to precisely track the location of the luminescent protein in the cell, the scientists additionally labeled it with a dye. In this way, they showed that the protein is evenly distributed and glows in healthy neurons. However, if the protein quality control is overstrained, the beetle protein makes clumps and no longer glows as strongly. The beetle protein therefore serves as a proteostasis sensor.

    The researchers then crossed the newly developed mouse line with mice that represent different neurodegenerative diseases. In mice showing signs of Alzheimer's disease, the luminescent protein formed clumps, signaling strong proteostasis disturbance. Interestingly, this was not the case in Chorea Huntington mice. Irina Dudanova relates, "The different results were quite surprising. When we had a closer look at the possible reasons, we found that both the misfolded proteins themselves and their location in the cell play an important role."

    While the misfolded protein in the Alzheimer's model forms deposits in the cell body, it clumps together in the cell nucleus in the Huntington’s mice. Accordingly, protein quality control and its capacity can vary greatly within a cell. "This shows how complex protein quality control is and how different its alterations can be in individual neurodegenerative diseases," explains Irina Dudanova.

    With the new mouse line, scientists now have a tool to specifically investigate this complexity – both in healthy and in diseased neurons. Irina Dudanova and her team plan to investigate other neurodegenerative diseases and to find out whether different cell types in the brain are affected at different rates. In addition, the mouse line could help to assess the effectiveness of different therapies for neurodegenerative diseases.

    CONTACT
    Dr. Christina Bielmeier
    Public Relations
    Max Planck Institute of Neurobiology, Martinsried
    Phone: 089 8578 - 3601
    E-mail: presse@neuro.mpg.de


    Wissenschaftliche Ansprechpartner:

    Dr. Irina Dudanova
    Research Group Leader Molecular Neurodegeneration
    Max Planck Institute of Neurobiology, Martinsried
    Phone: +49 89 8578 - 3542
    Email: idudanova@neuro.mpg.de


    Originalpublikation:

    Sonja Blumenstock*, Elena Katharina Schulz-Trieglaff*, Kerstin Voelkl, Anna-Lena Bolender,
    Paul Lapios, Jana Lindner, Mark S. Hipp, F. Ulrich Hartl, Rüdiger Klein and Irina Dudanova
    (*co-first)
    Fluc-EGFP reporter mice reveal differential alterations of neuronal proteostasis in aging and disease
    The EMBO Journal, online August 19th 2021


    Weitere Informationen:

    http://www.neuro.mpg.de/news/audio - Audio version of this press release
    http://www.neuro.mpg.de/dudanova - Website of the Research Group "Molecular Neurodegeneration"


    Bilder

    In healthy mouse neurons, the proteostasis sensor (in green) distributes evenly.
    In healthy mouse neurons, the proteostasis sensor (in green) distributes evenly.

    © MPI of Neurobiology / Blumenstock

    In cells that partially mimic Alzheimer's disease, the sensor protein clumps (cell in the middle, green dots), indicating a disruption in protein quality control.
    In cells that partially mimic Alzheimer's disease, the sensor protein clumps (cell in the middle, gr ...

    © MPI of Neurobiology / Blumenstock


    Merkmale dieser Pressemitteilung:
    Journalisten, Lehrer/Schüler, Studierende, Wissenschaftler, jedermann
    Biologie, Medizin
    überregional
    Forschungsergebnisse, Wissenschaftliche Publikationen
    Englisch


     

    Hilfe

    Die Suche / Erweiterte Suche im idw-Archiv
    Verknüpfungen

    Sie können Suchbegriffe mit und, oder und / oder nicht verknüpfen, z. B. Philo nicht logie.

    Klammern

    Verknüpfungen können Sie mit Klammern voneinander trennen, z. B. (Philo nicht logie) oder (Psycho und logie).

    Wortgruppen

    Zusammenhängende Worte werden als Wortgruppe gesucht, wenn Sie sie in Anführungsstriche setzen, z. B. „Bundesrepublik Deutschland“.

    Auswahlkriterien

    Die Erweiterte Suche können Sie auch nutzen, ohne Suchbegriffe einzugeben. Sie orientiert sich dann an den Kriterien, die Sie ausgewählt haben (z. B. nach dem Land oder dem Sachgebiet).

    Haben Sie in einer Kategorie kein Kriterium ausgewählt, wird die gesamte Kategorie durchsucht (z.B. alle Sachgebiete oder alle Länder).